Description: Mouse monoclonal antibody to Endobrevin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Endobrevin. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: VAMP8 Alternative Names: Vesicle-associated membrane protein 8; VAMP-8; Endobrevin; EDBEntrez Gene (Human): 8673SwissProt (Human): Q9BV40Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Endobrevin expression in Hela (A), THP1 (B), A431 (C) whole cell lysates.
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Immunohistochemical analysis of Endobrevin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.