Description: Mouse monoclonal antibody to CD273 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD273. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/4000), IH (1/50 - 1/200) Gene Symbol: PDCD1LG2 Alternative Names: B7DC; CD273; PDCD1L2; PDL2; Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273Entrez Gene (Human): 80380SwissProt (Human): Q9BQ51Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD273 expression in PDL2 transfected 293T (A) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 40-50 kD)
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  Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.