Description: Mouse monoclonal antibody to BBS4 Immunogen: Recombinant fusion protein of human BBS4. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: BBS4 Alternative Names: Bardet-Biedl syndrome 4 proteinEntrez Gene (Human): 585Entrez Gene (Mouse) : 102774SwissProt (Human): Q96RK4SwissProt (Mouse) : Q8C1Z7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BBS4 expression in Hela (A), U251 cell line (B), mouse heart (C), mouse kidney (D), rat heart (E), rat kidney (F) whole cell lysates.
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Immunohistochemical analysis of BBS4 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.