Description: Mouse monoclonal antibody to RAPTOR Immunogen: Recombinant fusion protein of human RAPTOR. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RPTOR Alternative Names: KIAA1303; RAPTOR; Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR)-scaffold proteinEntrez Gene (Human): 57521SwissProt (Human): Q8N122SwissProt (Mouse) : Q8K4Q0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RAPTOR expression in Hela (A), MCF7 (B), C2C12 (C), NIH3T3 (D), C6 (E) whole cell lysates.
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  Immunohistochemical analysis of RAPTOR staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.