Description: Mouse monoclonal antibody to CD276 Immunogen: Recombinant fusion protein of human CD276. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CD276 Alternative Names: B7H3; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD276Entrez Gene (Human): 80381SwissProt (Human): Q5ZPR3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD276 expression in 293 (A), A431 (B), U20S (C), LNCap (D), Hela (E), MCF7 (F) whole cell lysates.
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  Immunohistochemical analysis of CD276 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.