Description: Mouse monoclonal antibody to Caspase 8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 8. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: CASP8 Alternative Names: MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACHEntrez Gene (Human): 841SwissProt (Human): Q14790Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Caspase 8 expression in HepG2 (A), Jurkat (B), HL60 (C) whole cell lysates.
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Flow cytometric analysis of Jurkat cells using Anti-Caspase 8 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.