Description: Mouse monoclonal antibody to AMPK alpha 1 Immunogen: Recombinant fusion protein of human AMPK alpha 1. The exact sequence is proprietary. Clonality: Monoclonal Form: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PRKAA1 Alternative Names: AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1Entrez Gene (Human): 5562SwissProt (Human): Q13131Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AMPK alpha 1 expression in MCF7 (A), MDAMB468 (B) whole cell lysates.
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  Immunohistochemical analysis of AMPK alpha 1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.