Description: Mouse monoclonal antibody to MKK1 Immunogen: Recombinant fusion protein of human MKK1. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), FC (1/10 - 1/50) Gene Symbol: MAP2K1 Alternative Names: MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1Entrez Gene (Human): 5604Entrez Gene (Mouse) : 26395Entrez Gene (Rat) : 170851SwissProt (Human): Q02750SwissProt (Mouse) : P31938SwissProt (Rat) : Q01986Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MKK1 expression in Hela (A), A431 (B), PC12 (C), COS7 (D), NIH3T3 (E) whole cell lysates.
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  Flow cytometric analysis of Hela cells using Anti-MKK1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.