Description: Mouse monoclonal antibody to CDK5 Immunogen: Recombinant fusion protein of human CDK5. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CDK5 Alternative Names: CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunitEntrez Gene (Human): 1020Entrez Gene (Rat) : 140908SwissProt (Human): Q00535SwissProt (Rat) : Q03114Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK5 expression in SHSY5Y (A), SKBR3 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates.
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Immunohistochemical analysis of CDK5 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.