Description: Mouse monoclonal antibody to CD81 Immunogen: Recombinant fusion protein of human CD81. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/500) Gene Symbol: CD81 Alternative Names: TAPA1; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81Entrez Gene (Human): 975Entrez Gene (Rat) : 25621SwissProt (Human): P60033SwissProt (Rat) : Q62745Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD81 expression in A549 (A), Ramos (B), U87 MG (C) whole cell lysates.
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Immunohistochemical analysis of CD81 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.