Description: Mouse monoclonal antibody to Ki67 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ki67. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: IH (1/50 - 1/200) Gene Symbol: MKI67 Alternative Names: Antigen KI-67Entrez Gene (Human): 4288SwissProt (Human): P46013Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Ki67 expression in whole cell lysates.
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  Immunohistochemical analysis of Ki67 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.