Description: Mouse monoclonal antibody to MSH2 Immunogen: Recombinant fusion protein of human MSH2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/500) Gene Symbol: MSH2 Alternative Names: DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2Entrez Gene (Human): 4436SwissProt (Human): P43246Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of MSH2 expression in 293 (A), Hela (B) whole cell lysates.
-
Immunohistochemical analysis of MSH2 staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.