Description: Mouse monoclonal antibody to STAT3 Immunogen: Recombinant fusion protein of human STAT3. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/500 - 1/1000) Gene Symbol: STAT3 Alternative Names: APRF; Signal transducer and activator of transcription 3; Acute-phase response factorEntrez Gene (Human): 6774SwissProt (Human): P40763Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAT3 expression in CEM (A), Hela (B) whole cell lysates.
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Immunohistochemical analysis of STAT3 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.