Description: Mouse monoclonal antibody to AREB6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AREB6. The exact sequence is proprietary. Clonality: Monoclonal Form: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide. Dilution: WB (1/500 - 1/5000), FC (1/10 - 1/50) Gene Symbol: ZEB1 Alternative Names: AREB6; TCF8; Zinc finger E-box-binding homeobox 1; NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8Entrez Gene (Human): 6935SwissProt (Human): P37275Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AREB6 expression in A549 (A) whole cell lysates.
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Flow cytometric analysis of A549 cells using Anti-AREB6 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.