Description: Mouse monoclonal antibody to Prohibitin Immunogen: Recombinant fusion protein of human Prohibitin. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PHB Alternative Names: ProhibitinEntrez Gene (Human): 5245Entrez Gene (Mouse) : 18673Entrez Gene (Rat) : 25344SwissProt (Human): P35232SwissProt (Mouse) : P67778SwissProt (Rat) : P67779Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Prohibitin expression in 293 (A), MCF7 (B), HepG2 (C), NIH3T3 (D), rat liver (E) whole cell lysates.
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  Immunohistochemical analysis of Prohibitin staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.