Description: Mouse monoclonal antibody to AKT2 Immunogen: Recombinant fusion protein of human AKT2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: AKT2 Alternative Names: RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-betaEntrez Gene (Human): 208Entrez Gene (Mouse) : 11652Entrez Gene (Rat) : 25233SwissProt (Human): P31751SwissProt (Mouse) : Q60823SwissProt (Rat) : P47197Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of AKT2 expression in Jurkat (A), Hela (B), mouse brain (C), C6 (D) whole cell lysates.
-
Immunofluorescent analysis of AKT2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).