Description: Mouse monoclonal antibody to CDC25C Immunogen: Recombinant fusion protein of human CDC25C. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CDC25C Alternative Names: M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25CEntrez Gene (Human): 995SwissProt (Human): P30307Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC25C expression in HepG2 (A), K562 (B), MCF7 (C), Raji (D) whole cell lysates.
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Immunohistochemical analysis of CDC25C staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.