Description: Mouse monoclonal antibody to HSP47 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary. Clonality: Monoclonal Form: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SERPINH1 Alternative Names: CBP1; CBP2; HSP47; SERPINH2; Serpin H1; 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47Entrez Gene (Human): 871Entrez Gene (Mouse) : 12406SwissProt (Human): P50454SwissProt (Mouse) : P19324Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSP47 expression in rat heart (A) whole cell lysates.
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  Immunohistochemical analysis of HSP47 staining in human skin carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.