Description: Mouse monoclonal antibody to PAX6 Immunogen: Recombinant fusion protein of human PAX6. The exact sequence is proprietary. Clonality: Monoclonal Form: Mouse IgM kappa. Supplied in crude ascites with 0.01% sodium azide. Dilution: WB (1/500 - 1/4000), IF/IC (1/10 - 1/50) Gene Symbol: PAX6 Alternative Names: AN2; Paired box protein Pax-6; Aniridia type II protein; OculorhombinEntrez Gene (Human): 5080SwissProt (Human): P26367Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PAX6 expression in 293 (A) whole cell lysates.
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Immunofluorescent analysis of PAX6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).