Description: Mouse monoclonal antibody to MCM3 Immunogen: Recombinant fusion protein of human MCM3. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), FC (1/10 - 1/50) Gene Symbol: MCM3 Alternative Names: DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102Entrez Gene (Human): 4172Entrez Gene (Mouse) : 17215SwissProt (Human): P25205SwissProt (Mouse) : P25206Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MCM3 expression in CHO (A), NIH3T3 (B), Hela (C), HepG2 (D), C2C12 (E), PC12 (F) whole cell lysates.
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  Flow cytometric analysis of Hela cells using Anti-MCM3 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.