Description: Mouse monoclonal antibody to ME2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ME2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ME2 Alternative Names: NAD-dependent malic enzyme, mitochondrial; NAD-ME; Malic enzyme 2Entrez Gene (Human): 4200Entrez Gene (Mouse) : 107029SwissProt (Human): P23368SwissProt (Mouse) : Q99KE1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ME2 expression in HL60 (A), NIH3T3 (B), Jurkat (C) whole cell lysates.
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  Immunohistochemical analysis of ME2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.