Description: Mouse monoclonal antibody to CD73 Immunogen: Recombinant fusion protein of human CD73. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NT5E Alternative Names: NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73Entrez Gene (Human): 4907SwissProt (Human): P21589Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD73 expression in MDAMB231 (A) whole cell lysates.
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Immunohistochemical analysis of CD73 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.