Description: Mouse monoclonal antibody to Filamin A Immunogen: Recombinant fusion protein of human Filamin A. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IF/IC (1/10 - 1/50) Gene Symbol: FLNA Alternative Names: FLN; FLN1; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filaminEntrez Gene (Human): 2316Entrez Gene (Mouse) : 192176SwissProt (Human): P21333SwissProt (Mouse) : Q8BTM8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Filamin A expression in Hela (A), C6 (B), PC3 (C), COS7 (D), NIH3T3 (E) whole cell lysates.
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  Immunofluorescent analysis of Filamin A staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).