Description: Mouse monoclonal antibody to Galectin 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Galectin 3. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: LGALS3 Alternative Names: MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigenEntrez Gene (Human): 3958SwissProt (Human): P17931Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Galectin 3 expression in NCIH460 (A) whole cell lysates.
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Immunohistochemical analysis of Galectin 3 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.