Description: Mouse monoclonal antibody to CREB Immunogen: Recombinant fusion protein of human CREB. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CREB1 Alternative Names: Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1Entrez Gene (Human): 1385Entrez Gene (Mouse) : 12912Entrez Gene (Rat) : 81646SwissProt (Human): P16220SwissProt (Mouse) : Q01147SwissProt (Rat) : P15337Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CREB expression in A549 (A), Neuro2a (B), NIH3T3 (C), C6 (D) whole cell lysates.
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  Immunohistochemical analysis of CREB staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.