Description: Mouse monoclonal antibody to Histone H2A.X Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone H2A.X. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: H2AFX Alternative Names: H2AX; Histone H2AX; H2a/x; Histone H2A.XEntrez Gene (Human): 3014SwissProt (Human): P16104Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H2A.X expression in 293 (A), CEM (B), HepG2 (C), Jurkat (D), Hela (E), Raji (F) whole cell lysates.
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  Immunohistochemical analysis of Histone H2A.X staining in human thymus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.