Description: Mouse monoclonal antibody to nm23-H1 Immunogen: Recombinant fusion protein of human nm23-H1. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NME1 Alternative Names: NDPKA; NM23; Nucleoside diphosphate kinase A; NDK A; NDP kinase A; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated proteinEntrez Gene (Human): 4830SwissProt (Human): P15531Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of nm23-H1 expression in MCF7 (A) whole cell lysates.
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Immunohistochemical analysis of nm23-H1 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.