Description: Mouse monoclonal antibody to E Cadherin Immunogen: Recombinant fusion protein of human E Cadherin. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CDH1 Alternative Names: CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324Entrez Gene (Human): 999Entrez Gene (Mouse) : 12550SwissProt (Human): P12830SwissProt (Mouse) : P09803Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of E Cadherin expression in SW480 (A), MCF7 (B), WiDr (C), mouse stomach (D) whole cell lysates.
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  Immunohistochemical analysis of E Cadherin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.