Description: Mouse monoclonal antibody to PCNA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PCNA. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/500) Gene Symbol: PCNA Alternative Names: Proliferating cell nuclear antigen; PCNA; CyclinEntrez Gene (Human): 5111Entrez Gene (Mouse) : 18538Entrez Gene (Rat) : 25737SwissProt (Human): P12004SwissProt (Mouse) : P17918SwissProt (Rat) : P04961Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PCNA expression in C2C12 (A), C6 (B), Hela (C), L929 (D), MCF7 (E) whole cell lysates.
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  Immunohistochemical analysis of PCNA staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.