Description: Mouse monoclonal antibody to CDK4 Immunogen: Recombinant fusion protein of human CDK4. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CDK4 Alternative Names: Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3Entrez Gene (Human): 1019Entrez Gene (Mouse) : 12567SwissProt (Human): P11802SwissProt (Mouse) : P30285Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK4 expression in A431 (A), Hela (B), K562 (C), MCF7 (D), NIH3T3 (E) whole cell lysates.
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Immunohistochemical analysis of CDK4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.