Description: Mouse monoclonal antibody to ALDOC Immunogen: Recombinant fusion protein of human ALDOC. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG3. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: ALDOC Alternative Names: ALDC; Fructose-bisphosphate aldolase C; Brain-type aldolaseEntrez Gene (Human): 230Entrez Gene (Mouse) : 11676Entrez Gene (Rat) : 24191SwissProt (Human): P09972SwissProt (Mouse) : P05063SwissProt (Rat) : P09117Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ALDOC expression in HL60 (A), 293T (B), mouse brain (C), rat brain (D), mouse spleen (E) whole cell lysates.
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Flow cytometric analysis of HL60 cells using Anti-ALDOC Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.