Description: Mouse monoclonal antibody to PGP9.5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGP9.5. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: UCHL1 Alternative Names: Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1Entrez Gene (Human): 7345Entrez Gene (Rat) : 29545SwissProt (Human): P09936SwissProt (Rat) : Q00981Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PGP9.5 expression in 293T (A), NCIH1299 (B), PC12 (C) whole cell lysates.
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  Immunohistochemical analysis of PGP9.5 staining in human astroglioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.