Anti-Beta5-tubulin Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of Beta5-tubulin expression in 293 (A), CEM (B), Hela (C), Y79 (D), PC12 (E) whole cell lysates.
Immunohistochemical analysis of Beta5-tubulin staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Beta5-tubulin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Product Name	Anti-Beta5-tubulin Antibody
Cat No:	CQA9122
Source:	Mouse
Reactivity:	H, M, R
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

50 μl

$150

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Description: Mouse monoclonal antibody to Beta5-tubulin

Immunogen: Recombinant fusion protein of human Beta5-tubulin. The exact sequence is proprietary.

Purification: This antibody is purified through a protein G column.

Clonality: Monoclonal

Form: Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)

Gene Symbol: TUBB

Alternative Names: TUBB5; Tubulin beta chain; Tubulin beta-5 chain

Entrez Gene (Human): 203068

Entrez Gene (Mouse) : 22154

Entrez Gene (Rat) : 29214

SwissProt (Human): P07437

SwissProt (Mouse) : P99024

SwissProt (Rat) : P69897

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of Beta5-tubulin expression in 293 (A), CEM (B), Hela (C), Y79 (D), PC12 (E) whole cell lysates.
Immunohistochemical analysis of Beta5-tubulin staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Beta5-tubulin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

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