Description: Mouse monoclonal antibody to Cathepsin D Immunogen: Recombinant fusion protein of human Cathepsin D. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CTSD Alternative Names: CPSD; Cathepsin DEntrez Gene (Human): 1509SwissProt (Human): P07339Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cathepsin D expression in U251 MG (A) whole cell lysates.
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Immunohistochemical analysis of Cathepsin D staining in human lung adenocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.