Description: Mouse monoclonal antibody to Nucleophosmin Immunogen: Recombinant fusion protein of human Nucleophosmin. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NPM1 Alternative Names: NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; NumatrinEntrez Gene (Human): 4869SwissProt (Human): P06748Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Nucleophosmin expression in Hela (A), Jurkat (B), MCF7 (C), NIH3T3 (D) whole cell lysates.
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Immunohistochemical analysis of Nucleophosmin staining in human thymus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.