Description: Mouse monoclonal antibody to Von Willebrand Factor Immunogen: Recombinant fusion protein of human Von Willebrand Factor. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: VWF Alternative Names: F8VWF; von Willebrand factor; vWFEntrez Gene (Human): 7450SwissProt (Human): P04275Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Von Willebrand Factor expression in VWF protein (A) whole cell lysates.
-
Flow cytometric analysis of K562 cells using Anti-Von Willebrand Factor Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.