Description: Mouse monoclonal antibody to Alpha-fetoglobulin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-fetoglobulin. The exact sequence is proprietary. Clonality: Monoclonal Form: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: AFP Alternative Names: HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulinEntrez Gene (Human): 174SwissProt (Human): P02771Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Alpha-fetoglobulin expression in human fetal liver (A) whole cell lysates.
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Immunohistochemical analysis of Alpha-fetoglobulin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.