Description: Mouse monoclonal antibody to PAH Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAH. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PAH Alternative Names: Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenaseEntrez Gene (Human): 5053Entrez Gene (Mouse) : 18478SwissProt (Human): P00439SwissProt (Mouse) : P16331SwissProt (Rat) : P04176Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PAH expression in HepG2 (A), mouse liver (B), rat liver (C) whole cell lysates.
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  Immunohistochemical analysis of PAH staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.