Description: Mouse monoclonal antibody to ALDH1A1 Immunogen: Recombinant fusion protein of human ALDH1A1. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ALDH1A1 Alternative Names: ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolicEntrez Gene (Human): 216SwissProt (Human): P00352Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ALDH1A1 expression in NCIH460 (A) whole cell lysates.
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Immunohistochemical analysis of ALDH1A1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.