Description: Mouse monoclonal antibody to MITF Immunogen: Recombinant fusion protein of human MITF. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/500) Gene Symbol: MITF Alternative Names: BHLHE32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32Entrez Gene (Human): 4286Entrez Gene (Mouse) : 17342SwissProt (Human): O75030SwissProt (Mouse) : Q08874SwissProt (Rat) : O88368Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MITF expression in A431 (A), PC12 (B), NIH3T3 (C) whole cell lysates.
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Immunohistochemical analysis of MITF staining in human melanoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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