Description: Mouse monoclonal antibody to GCTM-2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GCTM-2. The exact sequence is proprietary. Clonality: Monoclonal Form: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide. Dilution: WB (1/1000 - 1/5000), IF/IC (1/10 - 1/50) Gene Symbol: PODXL Alternative Names: PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1Entrez Gene (Human): 5420SwissProt (Human): O00592Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GCTM-2 expression in A549 (A) whole cell lysates.
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Immunofluorescent analysis of GCTM-2 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).