Description: Rabbit polyclonal antibody to Nesprin 1 Immunogen: Recombinant protein corresponding to human Nesprin 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100) Gene Symbol: SYNE1 Alternative Names: C6orf98; KIAA0796; KIAA1262; KIAA1756; MYNE1; Nesprin-1; Enaptin; Myocyte nuclear envelope protein 1; Myne-1; Nuclear envelope spectrin repeat protein 1; Synaptic nuclear envelope protein 1; Syne-1Entrez Gene (Human): 23345Entrez Gene (Mouse) : 64009SwissProt (Human): Q8NF91SwissProt (Mouse) : Q6ZWR6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Nesprin 1 expression in mouse skeletal muscle (A) whole cell lysates.
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  Immunohistochemical analysis of Nesprin 1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Nesprin 1 staining in NIH3T3. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).