Description: Recombinant protein of human CD54. The exact sequence is proprietary. Immunogen: Recombinant protein of human CD54 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100) Gene Symbol: ICAM1 Alternative Names: Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54Entrez Gene (Human): 3383Entrez Gene (Mouse) : 15894Entrez Gene (Rat) : 25464SwissProt (Human): P05362SwissProt (Mouse) : P13597SwissProt (Rat) : Q00238Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD54 expression in A549 IFN-a treated (A), A549 IFN-r treated (B) whole cell lysates.
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  Immunohistochemical analysis of CD54 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of CD54 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).