Description: Rabbit polyclonal antibody to TRAF6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: TRAF6 Alternative Names: RNF85; TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85Entrez Gene (Human): 7189Entrez Gene (Mouse) : 22034Entrez Gene (Rat) : 311245SwissProt (Human): Q9Y4K3SwissProt (Mouse) : P70196SwissProt (Rat) : B5DF45Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of TRAF6 expression in Hela (A), mouse brain (B), rat kidney (C) whole cell lysates.
• 
  Immunofluorescent analysis of TRAF6 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).