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Anti-Ly-6G/C Antibody CPA8959
Product NameAnti-Ly-6G/C Antibody
Cat No: CPA8959
Source: Rat
Reactivity: M
Applications: IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$300
100 μl
$180
50 μl
$120
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Description: Rat monoclonal antibody to Ly-6G/C
Immunogen: Raised against granulocytes of mouse origin
Purification: The antibody was purified by affinity chromatography.
Clonality: Monoclonal (clone: RB6-8C5)
Form: Rat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Gene Symbol: Ly6c1; Ly6g
Alternative Names: Lymphocyte antigen 6C1; Ly-6C1; Lymphocyte antigen 6G; Ly-6G
Entrez Gene (Mouse) : 17067; 546644
SwissProt (Mouse) : P0CW02; P35461
Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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