Description: Mouse monoclonal antibody AF647 labled to CD25 Immunogen: Native purified human CD25. Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: 7G7B6) Conjugation: AF647 Form: Mouse IgG2a. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: IL2RA Alternative Names: Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD antigen CD25Entrez Gene (Human): 3559SwissProt (Human): P01589Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.