Description: Mouse monoclonal antibody FITC labled to CD38 Immunogen: Native purified human CD38. Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: HIT2) Conjugation: FITC Form: Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: CD38 Alternative Names: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD antigen CD38Entrez Gene (Human): 952SwissProt (Human): P28907Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube.
2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light.
3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells.
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant.
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant.
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.