Description: Mouse monoclonal antibody APC labled to CD158d Immunogen: NK3.3 cells and KIR2DL4-Ig fusion protein Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: mAb33) Conjugation: APC Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: KIR2DL4 Alternative Names: CD158D; KIR103AS; Killer cell immunoglobulin-like receptor 2DL4; CD158 antigen-like family member D; G9P; Killer cell inhibitory receptor 103AS; KIR-103AS; MHC class I NK cell receptor KIR103AS; CD158dEntrez Gene (Human): 3805SwissProt (Human): Q99706Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube.
2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light.
3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells.
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant.
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant.
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.