Description: Mouse monoclonal antibody Biotin labled to CD44 Immunogen: Native purified human CD44. Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: P2A1) Conjugation: Biotin Form: Mouse IgG2a. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: CD44 Alternative Names: LHR; MDU2; MDU3; MIC4; CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD antigen CD44Entrez Gene (Human): 960SwissProt (Human): P16070Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.