Description: Rabbit polyclonal antibody to Fibulin 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Fibulin 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: EFEMP1 Alternative Names: FBLN3; FBNL; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3; FIBL-3Entrez Gene (Human): 2202Entrez Gene (Mouse) : 216616SwissProt (Human): Q12805SwissProt (Mouse) : Q8BPB5SwissProt (Rat) : O35568Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Fibulin 3 expression in mouse lung (A), rat heart (B) whole cell lysates.
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  Immunohistochemical analysis of Fibulin 3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.